|
KMID : 0545120090190101271
|
|
Journal of Microbiology and Biotechnology
2009 Volume.19 No. 10 p.1271 ~ p.1279
|
|
|
Multi-Immunogenic Outer Membrane Vesicles Derived from an MsbBDeficient Salmonella enterica Serovar Typhimurium Mutant
|
|
Lee Sang-Rae
Kim Sang-Hyun
Jeong Kang-Jin
Kim Keun-Su
Kim Young-Hyun
Kim Sung-Jin
Kim Ek-yune
Kim Jung-Woo
Chang Kyu-Tae
|
|
|
Abstract
|
|
|
|
To develop low endotoxic and multi-immunogenic outer membrane vesicles (OMVs), a deletion mutant of the msbB gene in Salmonella enterica serovar Typhimurium (S. Typhimurium) was used as a source of low endotoxic OMV, and an expression vector of the canine parvovirus (CPV) VP2 epitope fused to the bacterial OmpA protein was constructed and transformed into the Salmonella ￥AmsbB mutant. In a lethality test, BALB/c mice injected intraperitoneally with the Salmonella ￥AmsbB mutant survived for 7 days, whereas mice injected intraperitoneally with the wild type survived for 3 days. Moreover, all mice inoculated orally with the ￥AmsbB mutant survived for 30 days, but 80% of mice inoculated orally with the wild type survived. The OmpA::CPV VP2 epitope fusion protein was expressed successfully and associated with the outer membrane and OMV fractions from the mutant S. Typhimurium transformed with the fusion protein-expressing vector. In immunogenicity tests, sera obtained from the mice immunized with either the Salmonella msbB mutant or its OMVs containing the OmpA::CPV VP2 epitope showed bactericidal activities against wild-type S. Typhimurium and contained specific antibodies to the CPV VP2 epitope. In the hemagglutination inhibition (HI) assay as a measurement of CPV-neutralizing activity in the immune sera, there was an 8-fold increase of HI titer in the OMVimmunized group compared with the control. These results suggested that the CPV-neutralizing antibody response was raised by immunization with OMV containing the OmpA::CPV VP2 epitope, as well as the protective immune response against S. Typhimurium in BALB/c mice.
|
|
|
KEYWORD
|
|
|
Outer membrane vesicle, S. Typhimurium, low endotoxicity, multi-immunogenicity, canine parvovirus
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
  
|